Recombinant Mouse Anti-B. burgdorferi OspA Antibody ( LA-2)
CAT#: PABZ-012
Recombinant Mouse Antibody ( LA-2) is capable of binding to B. burgdorferi OspA, expressed in Chinese Hamster Ovary cells (CHO). This murine monoclonal antibody defines an important protective B-cell epitope of OspA against which protective sera have strong levels of reactivity.
Published Data
Tested Data
Figure 1 Western blot analysis of B. burgdorferi-specific antibodies.
(A) mAbs, IS, and NMS used for reconstitution of scid mice, tested on strains B31 and ZS7 of B. burgdorferi. (B) Sera from individual scid mice reconstituted with the respective mAbs, IS, or NMS, tested on strain ZS7 (strain used for infection of mice) and on strain B31. The following mAbs or sera were used: mAb LA-5 (lane 1), mAb LA-2 (lane 2), mAb LA-10 (lane 3), mAb LA-21 (lane 4), anti-B31 IS (lane 5), anti-ZS7 IS (lane 6) and NMS (lane 7). (C) Amount (Ag/ml) of B. burgdorferi-specific antibody(ies) in reconstituted scid mice during the observation period (one representative experiment). Curves: 1, mAb LA-10; 2, mAb LA-21; 3, mAb LA-2; 4, mAb LA-5; 5, anti-B31; 6, PBS.
Schaible, U. E., Kramer, M. D., Eichmann, K., Modolell, M., Museteanu, C., & Simon, M. M. (1990). Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Proceedings of the National Academy of Sciences, 87(10), 3768-3772.
Figure 2 Histopathology in scid mice simultaneously inoculated s.c. with B. burgdorferi and i.p. with mAb LA-5 (a and c) or mAb LA-2 (b and d).
(a) Tibiotarsal joint of a scid mouse treated with LA-5. (b) Tibiotarsal joint of a scid mouse treated with the mAb LA-2. (c) Heart of a scid mouse treated with mAb LA-5. (d) Heart of a scid mouse treated with mAb LA-2.
Schaible, U. E., Kramer, M. D., Eichmann, K., Modolell, M., Museteanu, C., & Simon, M. M. (1990). Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Proceedings of the National Academy of Sciences, 87(10), 3768-3772.
❮
❯
SDS-PAGE
Figure 1 Recombinant Mouse Anti-B. burgdorferi OspA Antibody (PABZ-012) in SDS-PAGE
SDS-PAGE analysis of PABZ-012 in reduced (Lane 1) and non-reduced (Lane 2) conditions.
SEC-HPLC
Figure 2 Recombinant Mouse Anti-B. burgdorferi OspA Antibody (PABZ-012) in HPLC
HPLC analysis of PABZ-012 shows the purity is>95%.
❮
❯
Specifications
- Immunogen
- B. burgdorferi Outer surface protein A
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native B. burgdorferi OspA
- Species Reactivity
- B. burgdorferi
- Clone
- LA-2
- Applications
- WB 1:1000-1:5000
FuncS Assay-Dependent
IF 1:100-1:500
Neut Assay-Dependent
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody LA-2 has been reported in applications of WB, FuncS, IF, Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- Outer surface protein A; OspA; Outer-Surface Protein A; OSPA
- Gene ID
- 8319333
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABZ-012. Click the button above to contact us or submit your feedback about this product.
Perfect for Academic Pursuit
This is a great option for anyone in need of a trustworthy research-grade antibody.
Quick Shipping
The antibody was delivered on time, and its packaging guaranteed that it would arrive undamaged.
Definitely Justified the Price
Even though I was first dubious about the price, this antibody's performance has shown that it is well worth the money.
Q&As
-
What is the best way to maximize the binding of an antigen to an antibody?
A: Adjust parameters including buffer composition, incubation temperature, duration, and antibody concentration to increase binding efficiency.
-
How do I get rid of unnecessary or out-of-date antibodies?
A: Get rid of biohazardous waste according to the guidelines set forth by your organization. This usually indicates that a biohazard container ought to be used for the antibody's proper disposal.
-
Which precautions should be taken when handling antibodies?
A: Put on the appropriate protective clothing, such as gloves and a lab coat. If required, handle materials beneath a biosafety cabinet and follow your institution's established safety procedures for biological reagents.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABZ-012, RRID: AB_3111705)
Copy citation
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone LA-2"
- CAT
- Product Name
See other products for "B. burgdorferi OspA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-012 | Recombinant Mouse Anti-B. burgdorferi OspA Antibody (mAb184.1) | Neut | IgG |
| MRO-017CQ | Recombinant Mouse Anti-B. burgdorferi OspA Antibody (0551) | ELISA, IF | IgG |
| FAMAB-0187JF | Human Anti-B. burgdorferi OspA Recombinant Antibody (clone 3-24) | WB, ELISA, IHC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-012 | Recombinant Mouse Anti-B. burgdorferi OspA Antibody Fab Fragment (mAb184.1) | Neut | IgG |
| PFBZ-012 | Recombinant Mouse Anti-B. burgdorferi OspA Antibody Fab Fragment ( LA-2) | Neut, WB | IgG |
| FAMAB-0187JF-F(E) | Human Anti-B. burgdorferi OspA Recombinant Antibody (clone 3-24); Fab Fragment | FACS, IF, WB, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-012 | Recombinant Mouse Anti-B. burgdorferi OspA Antibody scFv Fragment (mAb184.1) | Neut | scFv |
| PSBZ-012 | Recombinant Mouse Anti-B. burgdorferi OspA Antibody scFv Fragment ( LA-2) | Neut, WB | scFv |
| FAMAB-0187JF-S(P) | Human Anti-B. burgdorferi OspA Recombinant Antibody (clone 3-24); scFv Fragment | FACS, IF, WB, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0040CQ | Recombinant Mouse Anti-B. burgdorferi ospA Antibody (LA-2) | ELISA | IgG2b |
| EPAF-0041CQ | Recombinant Mouse Anti-B. burgdorferi ospA Antibody (105.5) | WB | IgG1 |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
-2.png)
Application: IB, ELISA, FC, FuncS
-3-1.png)
Application: FC, IB, Block, Inhib, FuncS, ELISA, FACS, IP, IF
-2.png)
Application: WB, ELISA
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, Neut
Application: FC, IA, IF, IP, IHC, FuncS
-2.png)
Application: ELISA
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
